Fig. 4: UVB radiation inhibits ILC2-mediated lung inflammation via MC5R. | Nature Communications

Fig. 4: UVB radiation inhibits ILC2-mediated lung inflammation via MC5R.

From: Solar ultraviolet B radiation promotes α-MSH secretion to attenuate the function of ILC2s via the pituitary–lung axis

Fig. 4

a R5/+ and Mc5rfl/flR5/+ female mice (6–8 weeks) were intratracheally challenged with papain (4 µg/mouse) or PBS for 5 consecutive days with or without UVB irradiation of the eyes 24 h before day 1 and then sacrificed on day 6. b Percentage of eosinophils in the BALF (n = 6/group). c Representative H&E staining of lung sections (bars, 200 μm) (n = 6/group). d IL-5 and IL-13 in the BALF were detected by ELISA (n = 6/group). e Statistical analysis of the numbers of ILC2s in the lungs (n = 6/group). f Flow cytometric analysis of the percentages of IL-5+ and IL-13+ cells among lung ILC2s stimulated with PMA plus ionomycin and BFA for 4 h (n = 6/group). g WT female mice (6–8 weeks) were intratracheally challenged with papain (4 µg/mouse) or PBS for 5 consecutive days, treated with PBS or α-MSH (5 mg/kg) daily, and sacrificed on day 6. h Quantification of the eosinophil percentage in the BALF (n = 7, 6, 6, 6 from left to right). i Representative H&E staining of lung sections (bars, 200 μm) (n = 6/group). j ELISA was performed to measure the levels of IL-5 and IL-13 in the BALF (n = 7, 7, 6, 6 from left to right). k Statistical analysis of the numbers of ILC2s in the lungs (n = 7, 6, 6, 6 from left to right). l Flow cytometric analysis of IL-5 and IL-13 in lung ILC2s stimulated with PMA plus ionomycin and BFA for 4 h (n = 7, 6, 6, 6 from left to right). m ILC2s from R5/+ and Mc5rfl/flR5/+ female mice cultured with the culture supernatant of pituitary cells from WT female mice(6-8w) treated with or without UVB irradiation to the eyes. n α-MSH in the pituitary cell culture supernatant was measured by ELISA (n = 4/group); IL-5 and IL-13 in the supernatants were detected by ELISA (n = 4/sample). Each symbol represents an individual mouse (b–h, j–m). The data are representative of at least three independent experiments. The bars and error bars show the means ± SDs. For statistical analysis, the following tests were used. b–h, j–m Two-tailed unpaired Student’s t-test.

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