Fig. 2: Human iPS cell differentiation toward ECs.
From: Human blood vessel organoids reveal a critical role for CTGF in maintaining microvascular integrity
a Typical morphology of a human iPS colony, iPS derived ECs (iPS-ECs), and iPS-EC in vitro tube formation indicating their angiogenic potential are shown by bright field microscopy. Representative images of five independent experiments. b Immunofluorescence confocal image demonstrating the expression of pluripotency markers in human iPS cells and (c) EC-specific markers CD31, CD144 and ZO1 in iPS-ECs. Representative images of five independent experiments. d The expression of EC markers was confirmed by Western blot. N = 2 independent experiments were performed. Quantification of Basal Oxygen Consumption Rate (OCR) measured in iPS-ECs and HUVEC provided with either (e) glutamine, (f) palmitate, (g) pyruvate, or (h) glucose in the assay media. Three independent lines were assessed in n = 3 wells per assay per line. Values are presented as mean ± SEM; P values were calculated using a two-tailed Student’s t-test. (e: **p = 0.0072; f: **p = 0.0054). ns not significant. Source data are provided as a Source Data file.
