Fig. 2: sABE v3.22 achieves high DNA on-target editing efficiencies and enhanced resolutions.
From: A split and inducible adenine base editor for precise in vivo base editing
a Heat maps show the on-target DNA A-to-G editing efficiencies of ABE8e, sABE v3.22 induced with 100 nM rapamycin, and non-induced sABE v3.22 within the conventional A4-A8 activity window (numbered with 1 as the most PAM-distal position) at fourteen genomic loci. The control group was mock-transfected. Color brightness represents the mean value of three independent biological replicates. b Mean A-to-G editing efficiencies of ABE8e and sABE v3.22 at nineteen tested loci. Each dot represents the mean value of A-to-G editing efficiencies on adenine at a tested locus in a and Supplementary Fig. 5a. Dots are divided into bins based on the position of their corresponding adenine in the protospacer. A1-A3: n = 14, P = 0.0258; A4-A5: n = 20; A6-A8: n = 23, P < 0.0001; A9-A20: n = 62, P < 0.0001. c Comparison of the activity window and base editing efficiencies between ABE8e and sABE v3.22. d Representative sequence reads containing A-to-G conversions in ABE8e edited alleles (Top) or in sABE v3.22 edited alleles (Bottom) at Site 9. Colored nucleotides represent A-to-G conversions, and their colors represent if the reads contain single (green), double (yellow), or multiple (blue) A-to-G conversions. Numbers on the side represent the percentage of corresponding reads in total reads containing A-to-G conversions. e Bar plot showing the percentage of reads containing different numbers of base conversions in ABE8e edited alleles (Top) or in sABE v3.22 edited alleles (Bottom) at ten genomic loci. Bars represent the mean ± s.d of three individual biological replicates. b uses the unpaired two-tailed t-test; ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
