Fig. 3: Adriamycin–chromatin condensates co-localize with heterochromatin.
From: Chemical-induced phase transition and global conformational reorganization of chromatin
a, b U2OS cells were pulse-labeled with dUTP-Cy5 to label heterochromatin (a) or euchromatin (b), depending on the status of cell cycle. Cells were live-imaged at ×63 upon 1.5 μg/ml adriamycin treatment on Leica Thunder Imager microscope. As heterochromatin regions indicated by arrows, adriamycin accumulated primarily in heterochromatin, not euchromatin regions. Scale bar, 10 μm. c, d Adriamycin–chromatin condensates were co-localized with heterochromatin marker H3K9me3 (c) and transfected HP1-CFP (d). Scale bar, 10 μm. e, f Adriamycin condensates were partially co-localized with euchromatin marker H3K27ac (promoters and enhancers), but were localized mutual exclusively with H3K4me3 (promoters). Arrows indicate adriamycin condensates. Scale bar, 10 μm. g Adriamycin-induced nuclear p53 and adriamycin mainly exhibited mutually exclusive occupancy in nuclei. Arrows indicate the minority p53 that located within adriamycin condensates. Scale bar, 10 μm. Source data are provided as a Source data file. All experiments were repeated twice with similar results.
