Fig. 3: Abnormal peroxisome compartment architecture blocks lysine and histidine biosynthesis.
a Micrographs of pex11Δ S. japonicus cells grown in the yeast extract with supplements (YES) and post-shift to the Edinburgh minimal medium (EMM). b Cell morphology profiles of wild type and pex11Δ S. japonicus cells sampled at 7 h post-shift to EMM or EMM with indicated amino acid supplementation. Wild type grown in EMM, n = 249, 163 and 160 cells, respectively; pex11Δ in EMM, n = 345, 431 and 302 cells, respectively; pex11Δ in EMM with histidine, n = 278, 272 and 311 cells, respectively; pex11Δ in EMM with lysine, n = 328, 343 and 322 cells, respectively; and pex11Δ in EMM with lysine and histidine, n = 168, 261 and 333 cells, respectively. c Maximum Z-projection spinning disk confocal images of pex11Δ S. japonicus cells expressing Ctt1-mNeonGreen in indicated growth conditions. Supplemented amino acid L-lysine (Lys) is indicated in blue. Brightfield images are shown underneath the corresponding fluorescence channel images. d Catalase Ctt1-mNeonGreen protein abundance in pex11Δ S. japonicus cells normalised to the wild type, at time points 0 and 7 h, post-switch from YES to EMM, EMM supplemented with L-lysine or EMM supplemented with L-Histidine. Heatmap shows ratios between populational means of average cell fluorescence intensity. e Cell morphology profiles of pex11Δ (n = 277, 324 and 320 cells, respectively) and pex11Δ cells expressing an extra copy of GFP-Gpd1PTS1 (n = 324, 440 and 376 cells, respectively), sampled at 7 h post-shift to EMM. a, c Scale bars represent 5 μm. b, d, e Values are derived from three biological replicates. b, e Bars represent median values. p-values are derived from two-tailed unpaired t-test analysis. Source data are provided as a Source Data file.
