Fig. 3: Single-nucleus RNA-seq validation of osteogenic transcriptional programming at E15.5. | Nature Communications

Fig. 3: Single-nucleus RNA-seq validation of osteogenic transcriptional programming at E15.5.

From: Multimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis

Fig. 3

a Microsurgical dissection of age-matched littermates’ (pooled n = 3 biological triplicates) secondary palatal shelves in E13.5 and E15.5 mouse embryos was performed to isolate single nuclei suspensions for droplet-based Multiome RNA + ATAC-sequencing (10× Genomics). b Validation of bone marker expression profiles specifically within E15.5 secondary palate samples. c Further sub-clustering of mesenchyme-specific cell populations.

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