Fig. 4: RNAscope Multiplex for in vivo validation of spatiotemporal osteoblast marker mRNA expression in secondary palate tissues.
From: Multimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis
a Select osteogenic marker genes hybridized to track lineage-specific spatial patterns of differentiation: Runx2 (pre-osteoblast), Col1a1 (early functional osteoblast), Sparc (mature functional osteoblast), and Sost (osteocyte), in mid-palatal coronal sections via RNAscope Multiplex (*All in vivo validation experiments performed in biological and technical triplicate). b Proposed spatiotemporal osteogenic cell lineage activation sequence in developing secondary palate mesenchyme. *White arrows denote central ossification centers; Purple arrows indicate Sost+ cells identified in E15.5 secondary palate. All in situ experiments were performed in biological triplicate.
