Fig. 5: Spatial RNA sequencing (spRNA-seq) for the in situ resolution of the fusing palate’s transcriptome.
From: Multimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis
Mid-palatal coronal cross sections of whole embryo heads were placed on barcoded Visium slide. a In vivo clusters were defined from the whole embryo head, demonstrating spatial relationships and morphogenetic diversity of expression, further filtered for only those clusters encoded on the barcodes placed within the palate tissue in each respective section to identify top differentially expressed genes (DEG’s) b from E14.5 vs. E15.5 in the palate. *Denotes previously unreported palate-enriched genes identified in spRNA-seq. c Spatial gene expression feature plots for the three enriched genes identified. d Schematic summary. Colored circles correspond to 55 µm-diameter Visium transcriptomic resolution. Increased expression levels, delineated using the 10X Genomics Loupe Browser, are represented here with darker shades of green (Deup1), blue (Dynlrb2), or red (Lrrc23). The combined localization of these genes is indicated by overlapping concentric colored circles, the diameter of which does not correspond to degree of expression. nc nasal cavity, oc oral cavity, t tongue, mes midline epithelial seam; scale bar: 200 µm. All spatial RNA-seq experiments were performed in biological triplicate.
