Fig. 2: Absence of endothelial Sirt1 in male mice partially protects from diet-induced obesity and insulin resistance.

Mice were fed high-fat diet (HFD) for 12 weeks. a Bodyweight of Sirt1^fl/fl mice (n = 18) vs. KO mice (n = 20), *p <  0.05. b Lean mass of Sirt1^fl/fl mice (n = 12) vs. KO mice (n = 13), p = 0.001. c Fluid mass of Sirt1^fl/fl mice (n = 12) vs. KO mice (n = 13), p = 0.09. d Fat mass of Sirt1^fl/fl mice (n = 12) vs. KO mice (n = 13), p = 0.438. e Food intake of Sirt1^fl/fl mice (n = 10) vs. KO mice (n = 14), p = 0.601. f 24 h respiratory exchange ratio (RER) of Sirt1^fl/fl mice (n = 8) vs. KO mice (n = 8), p = 0.038. g Glucose tolerance test in Sirt1^fl/fl (n = 6) vs. KO (n = 8) mice, *p  < 0.05 (upper) with area under the curve, p = 0.023 (lower); and (h) Insulin tolerance test in Sirt1^fl/fl (n = 6) and KO (n = 5) mice, *p = 0.038. Data are shown as mean ± SEM. Two-tailed unpaired Student’s t test was used. In (a, f, g, and h), black line represents Sirt1^fl/fl -HFD mice, and blue line represents KO-HFD mice. In (b, c, d, e, and g), gray bar represents Sirt1^fl/fl-HFD mice, and blue bar represents KO-HFD mice. Each dark triangle represents one mouse. Sirt1^fl/fl Sirt1^flox/flox mice, KO E-Sirt1-KO mice. Source data are provided as a Source Data file.