Fig. 3: Small and large peritoneal macrophages are metabolically distinct.
From: Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infection
a Wanderlust trajectory analysis of total CD11b+CD11c− peritoneal exudate cells using immune marker (Ly6C, MHCII, F4/80, CX3CR1, CD206, TIM4) and metabolic protein expression. b Manual gating of MHCII+CD206+CPT1AHi macrophages, and representative histograms comparing their metabolic marker expression relative to total F4/80Hi macrophages. c gMFI of metabolic proteins in MHCII+CD206+, F4/80+TIM4− and F4/80+TIM4+ populations. One of five representative experiments, compared using one-way RM ANOVA (n = 4, mean ± SD). d SCENITH analysis of cavity macrophages using HPG incorporation following incubation with 1uM oligomycin (O), 100 mM 2-deoxy-d-glucose (2DG) or media controls (Co), one of two representative experiments shown with data compared using paired two-tailed t test (n = 4, mean ± SD), FAAO = Fatty acid/amino acid oxidation capacity. e Long-chain fatty-acid (BODIPY C16) uptake, mitochondrial polarization (TMRM) and mitochondrial mass (MitoTrackerDR) of peritoneal macrophage populations, data pooled from 3 experiments of n = 5 mice and compared using Wilcoxon two-tailed matched-pairs test. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Source data are provided as a Source Data file.
