Fig. 6: Shared metabolic phenotypes in alternatively-activated macrophages from different models of helminth infection.

a UMAP of macrophages from the livers of naïve or 16 week S. mansoni infected livers and (b) overlay of TIM4, PDL2 and RELMα expression. c Representative gating for naïve resident or S.m induced alternatively activated macrophages. d relative expression of metabolic marker normalized to mean expression on naïve Kupffer cells, n = 4–6 mice per group shown as individual data points and mean. e Gating for lung macrophages in naïve and N. brasiliensis infected mice, 10 days post-infection, and corresponding frequencies of Siglec F⁺ alveolar macrophages, n = 4 mice per group shown as individual data points and mean (e–g). f gMFI of alveolar and CD11b⁺ macrophage metabolic protein expression normalized to mean gMFI of respective population from naïve mice. g Representative gating for alternative activation markers and corresponding gMFI of metabolic markers segregated according to PDL2 or RELMα expression. h Fold change in metabolic markers across infections, relative to the matched naïve population from the same tissue. Data representative of one (Sm, n = 6) or two (Sm, n = 4/Hp, n = 2,5) independent experiments. Statistics calculated using one-way ANOVA with Tukey test for multiple comparisons (d) or two-tailed unpaired t test (e). ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Source data are provided as a Source Data file.