Fig. 5: Fc-mediated effector functions of the immune sera after vaccination with wild type or prefusion-stabilized mRNA constructs.

A, B Antibody-dependent neutrophil phagocytosis (ADNP). C, D Antibody-dependent cellular phagocytosis (ADCP). E, F Percentages of NK cells positive for CD107a. G, H Percentages of NK cells positive for MIP-1\(\beta\). I, J Antibody-dependent complement deposition (ADCD). Antibody response to wild-type LASV GPC vaccine (A, C, E, G, I) and prefusion stabilized LASV GPC vaccine (B, D, F, H, J). Ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001. One-way ANOVA with Tukey’s post-hoc test. A ns, P = 0.1145. B ns, P = 0.3029. C Between WT and PreF ns, P = 0.8455, between WT and control ns, P = 0.0955. D ns, P = 0.966. E Between WT and PreF ns, P = 0.9949. Between WT and control ns, P = 0.1120. Between PreF and control ns, P = 0.1326. F Between WT and PreF ns, P = 0.8805. Between WT and control ns, P = 0.9325. Between PreF and control ns, P = 0.9881. G ns, P = 0.6163. H Between WT and PreF ns, P = 0.7593. Between WT and control ns, P = 0.7045. Between PreF and control ns, P = 0.2990. I ns, P = 0.6545. J ns, P = 0.3827. For (A, B, E, F, G, and H), assays were run with PBMCs isolated from two separate donors. On all charts, the mean of the 5 animals per group and the standard deviation of the 5 animals per group is represented with error bars. For all figures, WT GPC vaccinated animals represented by a filled-in black circle. Prefusion GPC vaccinated animals represented by a filled-in green triangle. All non-vaccinated control animals represented but a filled-in purple diamond. For all figures, N = 5 animals per group. Source data are provided as a Source Data file.