Table 1 Different sets of SpCas9 variants (details with Addgene numbers can be found in the Materials and methods section and in Supplementary Data file 1)
Set A variants | Set B variants | CRISPRecise (Set C) variants | Additional in-between variants |
|---|---|---|---|
WT SpCas9 | WT SpCas9 | WT SpCas9 | Sniper SpCas9 |
eSpCas9-plus | Blackjack SpCas9 | Blackjack SpCas9 | HiFi SpCas9 |
B-SpCas9-HF1 | SpCas9-HF1 | B-Sniper SpCas9 | B-evoSpCas9-V495M |
B-HypaR-SpCas9 | B-HypaSpCas9 | B-HiFi SpCas9 | B-evoSpCas9-N515Y |
evoSpCas9 | eSpCas9 | B-evoSpCas9-E526K | |
B-evoSpCas9 | eSpCas9-plus | B-evoSpCas9-Q661R | |
B-HeFSpCas9 | SpCas9-HF1-plus | B-HeFSpCas9-A661R | |
SpCas9-HF1 | B-HeFSpCas9-A695Q | ||
B-eSpCas9 | B-HeFSpCas9-A848K | ||
HypaSpCas9 | B-HeFSpCas9-A926Q | ||
B-SpCas9-HF1 | B-HeFSpCas9-A1003K | ||
B-HypaSpCas9 | B-HeFSpCas9-A1060R | ||
HypaR-SpCas9 | |||
B-HypaR-SpCas9 | |||
evoSpCas9 | |||
B-evoSpCas9 | |||
HeFSpCas9 | |||
B-HeFSpCas9 |
