Fig. 7: mMORp viral transduction in human iPSC-derived MOR+ nociceptors. | Nature Communications

Fig. 7: mMORp viral transduction in human iPSC-derived MOR+ nociceptors.

From: Human OPRM1 and murine Oprm1 promoter driven viral constructs for genetic access to μ-opioidergic cell types

Fig. 7

Representative low (left, scale bar = 50 μm) and high (right, scale bar = 100 μm) magnification images of cultured human nociceptors treated with higher titer (1 × 1012 gc/mL, MOI: 2 × 108 [nociceptors], 1 × 108 [cardiomyocytes]) AAV.PHP.S-mMORp-eYFP virus (a), or an AAV.PHP.S-CAG-tdTomato virus (b). High magnification sample regions are denoted in lower magnification images via a white box. c, d Similar low (left) and high (right) magnification images of cultured nociceptors treated with lower titer mMORp-eYFP (1 × 109 gc/mL, MOI: 2 ×105 [nociceptors], 1 ×105 [cardiomyocytes], c) or CAG-tdTomato virus (d). Images of cultured human cardiomyocytes treated with high titer mMORp-eYFP (e) or CAG-tdTomato (f) viruses, with regions boxed in white denoting high magnification sample areas shown on the right. Images of cardiomyocytes treated with low titer mMORp-eYFP (g) or CAG-tdTomato (h) viruses, at both low (left) and high (right) magnification. Evidence of OPRM1 gene expression within cultured nociceptor cells is demonstrated in Supplementary Fig. 15.

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