Fig. 4: Depletion of LCs during weaning has no impact on the oral microbiota. | Nature Communications

Fig. 4: Depletion of LCs during weaning has no impact on the oral microbiota.

From: Langerhans cells shape postnatal oral homeostasis in a mechanical-force-dependent but microbiota and IL17-independent manner

Fig. 4

Langerin-DTR mice were treated with DT or PBS to deplete LCs during weaning, and the oral microbiota was sampled from adult mice for taxonomic analysis. A Total oral bacterial load determined by quantitative RT-PCR of the mean expression of the 16 S rRNA gene + SEM (n = 4 mice). p-value from two-tailed, unpaired t-test using GraphPad Prism. B Histograms represent the distribution of sequences in operational taxonomic units (OTUs) assigned to each family. C Principal coordinates (PCs) analysis of weighted UniFrac distances based on 16S rRNA of Langerin-DTR mice treated with DT or PBS. D Alpha diversity plot representing taxa richness in samples of both groups of mice. Data presented as standard boxplot, the median denoted as a central horizontal line in the box and the whiskers covering the data within ±1.5 IQR (n = 9 for PBS, n = 12 for DT). Taxonomic data was pooled from two independent experiments. Source data are provided as a Source Data file.

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