Fig. 2: Regulation of En expression in the stigmatophore of Drosophila melanogaster. | Nature Communications

Fig. 2: Regulation of En expression in the stigmatophore of Drosophila melanogaster.

From: Interlocking of co-opted developmental gene networks in Drosophila and the evolution of pre-adaptive novelty

Fig. 2

Expression of enD enhancer in wild type (a–c, e, g) or posterior spiracle mutant embryos (d, f, h). a enD-lacZ embryo double stained with anti-En (a’ green) and anti-ßGal (a” magenta). b, c e enD-lacZ embryos double stained with anti-ßGal (green) and either anti-Ct (b), anti-AbdB (c), or anti-Sal (e) in magenta. g enD-GFP upd0.43-lacZ embryo double stained with anti-GFP (g’ green) and anti-ßGal (g” magenta). d, f, h enD-lacZ embryos homozygous mutant for either Abd-BM1 (d), Df(2)5 sal-salr (f) or the upd locus deficiency Df(1)os1A (h), double stained with anti-ßGal (green) and anti-En (magenta). Panels b and g also show orthogonal sections to demonstrate Ct and upd0.43 are expressed in the internal spiracular chamber cells and enD-lacZ in the external stigmatophore cells. All embryos are st14. Scale bar: 20 µm.

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