Fig. 3: Expression and regulation of sal2.1 in the A8 segment. | Nature Communications

Fig. 3: Expression and regulation of sal2.1 in the A8 segment.

From: Interlocking of co-opted developmental gene networks in Drosophila and the evolution of pre-adaptive novelty

Fig. 3

a–c Expression of sal2.1-lacZ in wild type st13 embryos double stained with anti-ßGal (green, arrowheads) and anti-AbdB (a), anti-Sal (b), or anti-En (c) in magenta. d Expression of sal2.1-lacZ in Abd-BM5 mutant embryos lacking the m isoform. e Expression of sal2.1-PH-GFP in hh-lacZ embryos stained with anti-GFP (green) and anti-ßGal (magenta). f Expression of sal2.1-PH-GFP (green) in wild type embryos stained with anti-En (magenta). g, h Ectopic activation with the ectoderm-specific line 69B-Gal4 of the UAS-Abd-Bm (g) or UAS-Abd-Br isoform (h) in sal2.1-PH-GFP embryos stained with anti-GFP (green) and anti-En (magenta). Arrowheads in a–e point at anterior A8 cells, asterisk in panel d indicates hindgut expression. Scale bar: 50 µm.

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