Fig. 5: Expression of posterior spiracle network genes in the testis terminal region. | Nature Communications

Fig. 5: Expression of posterior spiracle network genes in the testis terminal region.

From: Interlocking of co-opted developmental gene networks in Drosophila and the evolution of pre-adaptive novelty

Fig. 5

a–e Expression of enD, sal2.1 and upd0.43 enhancers in the HCC at the testis terminal region. (a) enDds-GFP (green) is expressed in the HCCs holding the 64 sperm head bundles. Sperm tails labelled with Axo49 are shown in magenta, and sperm heads are in yellow with DAPI. (a’) shows only GFP and DAPI stainings and a close-up of an HCC holding the sperm heads. b The HCC labelled in green with enDds-GFP can be distinguished by the formation of an actin basket [labelled with phalloidin (magenta or white)] holding the 64 sperm head bundles (yellow). c enDds-GFP is active in HCC cells which also express low levels of endogenous Engrailed (magenta). d sal2.1-mCherry is active in cells expressing low levels of endogenous Sal protein. e upd0.43-lacZ (magenta) is active in a subset of HCC expressing Sal (green). Wild type enD0.4-mCherry reporter expression in HCCs (f). Reporter expression is maintained in enD0.4 when the putative Abd-B DNA-binding sites are mutated (g), or when the putative STAT sites are mutated (h). In (a, b, d–h) DAPI staining is yellow. Scale bar: 20 µm.

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