Fig. 6: Expression of Sal and En in the testis of three dipteran species. | Nature Communications

Fig. 6: Expression of Sal and En in the testis of three dipteran species.

From: Interlocking of co-opted developmental gene networks in Drosophila and the evolution of pre-adaptive novelty

Fig. 6

Testis terminal region of D. melanogaster (a, b), D. virilis (c, d) and whole testis of E. balteatus (e, f) stained with anti-Engrailed and anti-Spalt (a, c, e) or anti-Spalt, and the axoneme marker anti-Axo49 (b, d, f). D. virilis testes are larger but similar in shape to those of D. melanogaster and have a similar expression of En and Sal in the terminal region (a, c) where the sperms coil and individualise (b, d). The testis morphology of E. balteatus is completely different and expresses neither En nor Sal (e). Axoneme staining with anti-Axo49 and DAPI reveals the formation of shorter sperms in E. balteatus that do not coil (f). DAPI DNA staining in yellow. Scale bar: 100 µm.

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