Fig. 4: Molecular mechanism of TASL recognition by PHT1. | Nature Communications

Fig. 4: Molecular mechanism of TASL recognition by PHT1.

From: Molecular basis of TASL recruitment by the peptide/histidine transporter 1, PHT1

Fig. 4

a Close-up view of the central binding pocket (top) and C-domain interface of PHT1 (bottom), in the AF2 model of PHT1-TASL. Selected residues are shown as sticks and yellow dashes indicate hydrogen bonds (cutoff at 3.2 Å). b Thermal stability of PHT1 screened with different TASL peptides at a concentration of 50 µM. Changes in the melting temperature of PHT1 in the presence of a peptide as compared to the melting temperature of PHT1 alone (ΔTm) are shown. Data represent the mean ± SD of three biological replicates. c Pull-down assay using Twin-Streptavidin-tagged PHT1 and GFP-His-tagged TASL mutants at the interface residues. Shown data are representative of two independent experiments with similar results. d Pull-down assay using Twin-Streptavidin-tagged PHT1 mutants and GFP-His-tagged TASL at the interface residues. Shown data are representative of two independent experiments with similar results. Source data for relevant information are provided as a Source Data file.

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