Fig. 1: A CRISPR screen identified KEAP1 deletion abrogated LPS and IFN-γ induced pro-inflammatory activation in macrophages. | Nature Communications

Fig. 1: A CRISPR screen identified KEAP1 deletion abrogated LPS and IFN-γ induced pro-inflammatory activation in macrophages.

From: Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors

Fig. 1

a A schematic diagram of the pooled CRISPR screen of metabolic genes in THP1-induced macrophages (tMAC). b A volcano plot displaying sgRNA-targeted genes enriched in the CD80high (blue) and CD80low (red) populations. c The protein level of KEAP1 in WT and KEAP1-deficient tMACs. d, e Flow cytometry plots and quantification of CD80 expression on WT and KEAP1-deficient tMACs after LPS and IFN-γ stimulation for 0, 2, 8, and 24 h. e n = 3 biologically independent samples. Statistics by two-way ANOVA test. (8 h, P < 0.0001; 24 h, P < 0.0001) f qRT-PCR analysis of IL6, IL1B, CXCL9, and CXCL10 expression in WT and KEAP1-deficient tMACs after LPS and IFN-γ stimulation at different time points (n = 3 biologically independent samples). Statistics by two-way ANOVA test. e, f The data were displayed as mean ± SD. Source data are provided as a Source Data file.

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