Fig. 2: ACOD1 deletion promoted pro-inflammatory activation in THP1-induced macrophages.

a The relative expression of ACOD1 in WT and sgACOD1 transduced THP1-induced macrophages (tMAC) with LPS and IFN-γ stimulation at the indicated time points (n = 3 biologically independent samples). Statistics by two-way ANOVA test. (Day 0, P < 0.0001; Day 1, P < 0.0001; Day 2, P = 0.4529) b The protein level of ACOD1 in WT and sgACOD1-transduced cells after LPS and IFN-γ stimulation for 24 h. This experiment has been repeated for three times with similar results. c, d Flow cytometry plots and quantification of CD80 expression in unstimulated, WT, and sgACOD1 transduced tMACs with indicated treatments (d, n = 3 biologically independent samples). Statistics by two-way ANOVA test. The tMACs were stimulated by 50 ng/mL LPS and 50 ng/mL IFN-γ for 24 h, then withdrawn from the stimulation and further cultured for 24 h (Day 1) or 48 h (Day 2). e qRT-PCR for mRNA expression of pro-inflammatory genes in WT and sgACOD1 transduced tMACs after LPS and IFN-γ stimulation at different time points (n = 3 biologically independent samples). Statistics by two-way ANOVA test. (IL6: 2 h, P = 0.0211; 8 h, P < 0.0001; 24 h, P < 0.0001. CXCL9: 8 h, P < 0.0001; 24 h, P = 0.9755. CXCL10: 8 h, P < 0.0001; 24 h, P = 0.0026. CXCL11: 8 h, P < 0.0001; 24 h, P = 0.0075.) a, d, e Data was shown as mean ± SD. Source data are provided as a Source Data file.