Fig. 5: ACOD1 deletion decreased nucleolar NRF2 protein expression and its activity in iMACs. | Nature Communications

Fig. 5: ACOD1 deletion decreased nucleolar NRF2 protein expression and its activity in iMACs.

From: Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors

Fig. 5

a qRT-PCR for mRNA expression of NRF2 in WT and ACOD1-/- iMACs after LPS and IFN-γ stimulation for 2, 8, or 24 h (n = 3 biologically independent samples). Statistics by two-way ANOVA test. b qRT-PCR for mRNA expression of NRF2 downstream genes in WT and ACOD1-/- iMACs after LPS and IFN-γ stimulation for 24 h (n = 3 biologically independent samples). Statistics by two-way ANOVA test. (SOD2, P = 0.0029; HMOX1, P < 0.0001; GCLM, P = 0.009; NQO1, P = 0.0092; GSR, P = 0.0267) c, d Representative confocal images and quantification of the NRF2 protein in WT and ACOD1-/- iMACs after LPS and IFN-γ stimulation for 2 h, fluorescence intensity (FI) of single cells was counted (d, n = 60 cells from 3 biologically independent samples each group). Statistics by two-way ANOVA test. (2 h, P < 0.0001; 8 h, P < 0.0001) This experiment has been repeated for three times with similar results. Representative confocal images were obtained using the Olympus FV3000 microscope. a, b, d Data was shown as mean ± SD. Source data are provided as a Source Data file.

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