Fig. 7: PZP-Epithelial cells interaction alters gene expression. | Nature Communications

Fig. 7: PZP-Epithelial cells interaction alters gene expression.

From: Stromal heterogeneity may explain increased incidence of metaplastic breast cancer in women of African descent

Fig. 7

a IL-6 expression was detected only when luminal and PZP cells were co-cultured. R&D systems cytokine/chemokine array was used to identify secreted factors by epithelial and PZP cells either alone or together (50% each). Expression of genes in PZP (KTB32, KTB40, KTB42), epithelial (KTB34, KTB39), and co-culture of PZP and epithelial cell lines. b IL-6 (n = 3), KTB34 + 32*p < 0.0001, KTB34 + 40*p = 0.0018; KTB34 + 42*p = 0.0018; KTB39 + 32*p = 0.0003; KTB39 + 40*p = 0.007; KTB39 + 42*p = 0.0016. c TAGLN (n = 3), KTB34 + 32*p = 0.0004; KTB34 + 40*p = 0.0011; KTB34 + 42*p < 0.0001; KTB39 + 32*p = 0.0001; KTB39 + 40*p < 0.0001; KTB39 + 42*p = 0.0002. d WISP1 (n = 3), KTB34 + 42*p = 0.0218; KTB39 + 42*p = 0.0456. e TNC (n = 3), KTB34 + 32*p = 0.0401; KTB34 + 42*p = 0.0353; KTB39 + 32*p = 0.0023; KTB39 + 40*p = 0.0158; KTB39 + 42*p = 0.0005. f CSF1 (n = 3), KTB34 + 40*p = 0.0059; KTB34 + 42*p = 0.0112; KTB39 + 32*p = 0.0135; KTB39 + 40*p = 0.0016; KTB39 + 42*p = 0.0008. g SPP1 (n = 3), KTB39 + 32*p = 0.0348; KTB39 + 40*p = 0012. h IL-33 (n = 3), KTB34 + 32*p = 0.0269, KTB34 + 40*p = 0.0164; KTB34 + 42*p = 0.0045; KTB39 + 32*p = 0.0078; KTB39 + 40*p = 0.0017; KTB39 + 42*p = 0.0005. Statistical significance (p values) was determined by comparing KTB32/40/42 (PZP cells), KTB34/KTB39 (epithelial cells) and respective co-cultured PZP + epithelial cells as indicated in the figure. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by One-way ANOVA. All the data points are shown as mean ± SEM. Source data are provided as a Source Data file.

Back to article page