Fig. 4: Heterogeneity of cell types and developmental trajectories of single cells in the proximal-lateral ectoderm of E7.5 embryo. | Nature Communications

Fig. 4: Heterogeneity of cell types and developmental trajectories of single cells in the proximal-lateral ectoderm of E7.5 embryo.

From: Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation

Fig. 4

a–f t-SNE plots showing the heterogeneous cell clusters in position-9R2 and -8R2. Cells are annotated by position (a, c, e) and cell type (b, d, f). Dashed arrows (in a, c, e) denote the heterogeneous cell clusters in position-9R2 and -8R2 versus other lateral positions. Panel a–d showed the single cells of proximal-lateral ectoderm positions in sections 9 (a, b) and 8 (c, d). Panel e, f showed the single cells of proximal-lateral ectoderm, primitive streak, and mesoderm positions in section 9. g The spatial distribution of single cells in the Annulus Model of ectoderm at Geo-seq section 8/9. h–k t-SNE plots (h, j) and the molecular trajectories (i, k) of single cells (imputed using the population tracing algorithm) at position-8R2/9R2 (h, i) and position-8L2/9L2 (j, k) in E7.5–E8.5 embryos. Developmental time points (stage) and cell types (see legend) are indicated in the t-SNE plots. Cell types in E7.75–E8.5 embryos are annotated according to the ‘Gastrulation Atlas’.

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