Fig. 2: C3N nanodots reduce Aβ42-aggregation-induced cytotoxicity.
From: C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer’s disease
Neurons were cultured with/without Aβ42 peptides for 24 h. Cytotoxicity of Aβ42 aggregates in the presence/absence of different concentrations of C3N nanodots for 24 h to primary neurons was assayed by a CCK8 (P < 0.0001, P < 0.0001, P = 0.0300, 0.0114, and 0.0009, respectively) and b LDH-release (P < 0.0001, P < 0.0001, P = 0.0312, 0.0065, and 0.0035, respectively), n = 3 independent experiments. Statistical significance was determined by one-way ANOVA in (a–c) with p < 0.05 considered statistically significant. c, d Live/dead staining experiments to examine whether C3N nanodots alleviate the cytotoxicity of neurons induced by Aβ42 peptides. n = 5 independent experiments. Statistical significance was determined by unpaired Student’s t test (two-tailed) with P < 0.05 considered statistically significant (P < 0.0001, P = 0.0005). d Photomicrographs of live/dead assay showing live (green cell body) and dead (red nuclei) cells in each group. e Morphology of cells in each group was observed under SEM. The images are from one experiment representative of three independent experiments with similar results. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ***P < 0.0001 vs 50 μM Aβ42 group. n.s. = not significant. Source data are provided as a Source data file.
