Fig. 5: Phenotypes of normal colony and small colony variants in J strains.

a Growth rates of J strains in Yeast Extract-Peptone-Dextrose (YPD, left panel) and Yeast Extract-Peptone-glycerol (YP-glycerol, right panel) broth. Cells were incubated in respective media at 30° C. Growth of strain J5 in YPD was intermediate to that of strains J1-J4 (normal colony variants) and J6-J10 (small colony variants). J5 grouped with J6-J10 by not growing in YP-glycerol. Note the difference in Y-axis scales for the graphs. Data presented are mean ± standard error of mean from triplicate experiments. b Morphology of J strains on eosin Y plates. Normal colony variant strains were light pink, whereas small colony variant strains were dark pink to purple. Strains J1-J4 and J5-J10 stained as normal and small colony variants, respectively. Control strain C. glabrata BG2 stained as a normal colony variant. c Cell sizes of J strains, as measured by FACS. Cells in stationary phase were washed prior to measurement. Forward versus scatter (FSC and SSC) density plot was performed, and the cell population of interest was identified with polygon gating that encompassed at least 95% of all cell population. Box and whisker plots of FSC-A are used to represent cell sizes. The upper and lower whiskers represent maximum and minimum values, respectively; the hinges of the box represent 25% to 75% percentiles and the horizontal line within the box represents median values. The data shown are combined FSC-A data from 3 independent experiments. The mean rank of FSC-A of the index strain J1 was compared with those of the other J strains, and p-values determined using Kruskal-Wallis test with Dunn’s multiple comparisons. The mean rank of J1 FSC-A was significantly higher than those of J5 (mean rank difference of 54,773), J6 (60,758), J7 (57,307), J8 (61,710), J9 (64,492) and J10 (76,495); *p-values < 0.0001.