Fig. 4: Combination of high fat diet with streptozotocin treatment induces adipose tissue loss.
From: Glycerol contributes to tuberculosis susceptibility in male mice with type 2 diabetes
Control diet mice (CD), mice treated with streptozotocin (STZ), mice fed high fat diet (HFD) and mice fed high fat diet and treated with streptozotocin (HFD-STZ) infected with ~100 CFU Mtb Erdman for 8 weeks were compared to uninfected age-matched mice. a Mass of perigonadal white adipose tissue (pgWAT) (n = 4 mice for STZ, n = 5 mice for HFD and n = 6 mice for the others). b Average cell size of adipocytes in pgWAT (left) (n = 4 mice for STZ and HFD-STZ and n = 6 mice for the others) and representative images of H&E-stained pgWAT of infected mice (right). Scale bar, 100 µm. c Content of TG in pgWAT (n = 3 mice for STZ and n = 6 mice for the others). d Plasma levels of glycerol (n = 6 mice) and free fatty acids (FFA) (n = 5 mice for STZ and HFD, n = 6 mice for the others). Uninfected CD, HFD, STZ or HFD-STZ mice were intraperitoneally injected with PBS or 1 mg/kg insulin and after 15 min blood and pgWAT were harvested. e De novo lipogenesis was analyzed by measuring P-ACLY, total ACLY and FASN (n = 3 mice for STZ and HFD-STZ, n = 4 mice for the others). f Lipolysis was analyzed by measuring ATGL, P-HSL and total HSL (n = 3 mice for STZ and HFD-STZ, n = 4 mice for the others). Equal number of samples from each group were run on each gel/blot and they were run in parallel. Differences were expressed as fold change to the CD group to compare between different blots and using β-actin as loading control that was probed in the same gel or run in parallel in the case of ATGL. Data are expressed as mean ± SD. Statistical analysis was performed by One-Way ANOVA for uninfected, 8 weeks p.i., untreated or insulin separately, *P < 0.05, **P < 0.01 and ***P < 0.001 (p value from left to right = (a): all < 0.0001; (b): all uninfected < 0.0001 infected 0.0247 and 0.0001 the rest; (c): 0.0034; (d): left all < 0.0001, right 0.0127, 0.0236, 0.0588; (e): middle all < 0.0001, right 0.0190, 0.0005, 0.0040; (f): 0.0421, 0.0497). Source data are provided as a Source Data file.
