Fig. 3: Touch-induced hyponasty causes cytosolic Ca2+ increase in the touched leaf.
From: Mechanodetection of neighbor plants elicits adaptive leaf movements through calcium dynamics
a Fluorescence induction in the leaf of untouched (Control) or touch-treated (Touch) leaves after 10 min of touch using the fluorescent cytosolic calcium biosensor UBQ10pro::GCaMP3. b Six different positions were used to measure the GCaMP3 fluorescence in leaf upon control or touch treatment. Gray line represents the transparent tag. c Time course of GCaMP3 fluorescence intensity in leaf tip (position 1), primary vein of the lamina (position 2 and 3), lamina-petiole junction (position 4), in the middle of the adaxial site of the petiole (position 5), and in the adaxial site of the petiole base (position 6) upon control (black line) and touch (green line) treatment. For “Control” treatment n = 10 biologically independent replicates while for “Touch” treatment n = 11 biologically independent replicates. Touch treatment started at t = 2 min (120 sec) and fluorescence was followed for 25 minutes. Touch was induced by a gently positioned transparent tag next to the leaf. Data represent mean ± SE. Scale bar corresponds to 1 mm. Leaf drawing in b was created with Biorender.
