Fig. 4: GLRs are required for the full touch-induced hyponasty response with the involvement of [Ca2+]cyt.
From: Mechanodetection of neighbor plants elicits adaptive leaf movements through calcium dynamics
a Differential petiole angle of Col-0 compared to glr3.3glr3.6 double mutant after 24 h of touch treatment. n = 10 biologically independent replicates (b, c). The touch response of the glr3.2glr3.3glr3.6 triple mutant is significantly reduced compared to Col-0 after 24 h of touch treatment. n = 8 biologically independent replicates. d Picture of fluorescence induction in the leaf after 12 min of untouched (Control) or touch treatment (Touch) using the fluorescent cytosolic calcium biosensor UBQ10pro::GCaMP3 and glr3.1glr3.3glr3.6 with UBQ10pro::GCaMP3. e Relative GCaMP3 fluorescence (ΔF/F touch treatmenttx−ΔF/F controlaveragetx) time course in leaf tip (Position 1) touch treatment in Col-0 and glr3.1glr3.3glr3.6. d, e n = 12 biologically independent replicates for UBQ10pro::GCaMP3 and n = 15 biologically independent replicates for glr3.1glr3.3glr3.6 with UBQ10pro::GCaMP3. Touch treatment started at t = 2 min (120 sec) and fluorescence was followed for 20 minutes. Touch was induced by a gently positioned transparent tag next to the leaf. Black dots represent the individual data. Data represent mean ± SE. Different letters indicate significant differences (two-way ANOVA with Tukey’s post hoc test; p < 0.05). Scale bar corresponds to 4 mm.
