Fig. 9: Blinded pathologist subjective stain quality survey. | Nature Communications

Fig. 9: Blinded pathologist subjective stain quality survey.

From: Deep learning-enabled realistic virtual histology with ultraviolet photoacoustic remote sensing microscopy

Fig. 9

a Mean scores for each image quality metric provided by each pathologist for breast tissue virtual histology images of n = 15 independent specimens and n = 15 independent H&E-stained breast tissue frozen section specimens, with swarm plots showing the distribution of individual scores. Bars indicate mean ± SEM. b Box plots summarizing results for each histological imaging method and image quality metric using the inter-observer average image scores. Box center line and limits represent median and lower/upper quartiles, respectively. Whiskers represent lower/upper extrema, and no outliers are present. Diamond markers and associated errors bars report mean ± SEM. P values were determined using a right-tailed Wilcoxon rank sum test, without adjustments for multiple comparisons. SEM standard error of the mean, HD hematoxylin detail, ED eosin detail, SQ overall stain quality, HS histological stain, VS virtual stain. c Representative frozen section H&E-stained breast tissue brightfield microscopy. Scale bar: 50 μm. Source data are provided as a Source Data file.

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