Fig. 4: Statistical results for the mixed hexamers. | Nature Communications

Fig. 4: Statistical results for the mixed hexamers.

From: Determining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex

Fig. 4

a Probabilities of KaiC hexamers in different conformational patterns for the mixed sample data set (19,858 rings). Given the large number of hexamers in each pattern (>100 particles), the relative errors are small (<10%). b Normalized correlation function of the mixed sample. Dots are calculated from the experimental data while the solid line is from the fitting by the Ising model. About \({10}^{6}\) subunit pairs are used to calculate \(\widetilde{C}(x)\), so the error is ~\({10}^{-3}\), too small to show in the figure. The fitting parameters are: \({J}_{{mix}}=0.086,{B}_{{mix}}=-0.16\). Comparison between the mixed experimental data and model results with the no-mixing scenario (c), the fully-mixed scenario (d), and\(\,{q}_{l}\) treated as free parameters with \(\lambda=0.1,J=0.14\)(e).

Back to article page