Fig. 3: VE-cadherin is not restricted to endothelial cell junctions. | Nature Communications

Fig. 3: VE-cadherin is not restricted to endothelial cell junctions.

From: VE-cadherin in arachnoid and pia mater cells serves as a suitable landmark for in vivo imaging of CNS immune surveillance and inflammation

Fig. 3

Confocal imaging of 100 μm thick brain and spinal cord sections of a healthy VE-cadherin GFP knock-in reporter mouse and 20 μm thick brain and spinal cord sections of wild-type C57BL/6 J. PECAM-1 (red) and/or VE-cadherin (yellow) immunostaining was performed. DAPI (blue) stains the nucleus. Magnification ×40. A–D XY MIP of the overview (left) and zoomed-in (right) representative images of the meningeal layers of the A–C spinal cord and B–D brain. A, B Endogenous VE-cadherin GFP+ signal in the endothelial AJs of the meningeal and parenchymal blood vessels is seen in green. Additional VE-cadherin GFP signal is visible outside the blood vessel walls (red) on the surface of the brain and spinal cord. C, D VE-cadherin staining in the endothelial AJs of the meningeal and parenchymal blood vessels is seen in yellow. Additional VE-cadherin staining (yellow) is visible outside the blood vessel walls (red) on the surface of the brain and spinal cord. Images are representative of a total of three mice imaged in a total of three experiments.

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