Fig. 6: Proposed mechanism of POD-like catalysis of Fmoc–F–F (His) and key structural parameters.
From: Histidine modulates amyloid-like assembly of peptide nanomaterials and confers enzyme-like activity
Seven reaction states mediated by POD-like catalysis of Fmoc–F–F (His) was proposed. The whole process can be divided into three major steps: The adsorption of H2O2 and (H+ + TMB) molecules on Fmoc–F–F (His) (states 1–3); Oxidation reaction of the first TMB molecule oxidized by H2O2* under acidic conditions, producing HO*, H2O*, and oxTMB* (states 4–5); Oxidation of second TMB molecule by second HO* under acidic conditions, producing H2O* and oxTMB* (states 6–7). The corresponding binding energy of each step can be seen in Supplementary Fig. 33.
