Fig. 2: Enhanced adhesion of PAV-engineered K562 cells to vascular endothelial cells. | Nature Communications

Fig. 2: Enhanced adhesion of PAV-engineered K562 cells to vascular endothelial cells.

From: Nongenetic surface engineering of mesenchymal stromal cells with polyvalent antibodies to enhance targeting efficiency

Fig. 2

a Schematic diagram of the bottom-up assembly of MAV or PAV on the cell membrane. b Confocal fluorescence image of PAV-engineered K562 cells. c Flow cytometric analysis of the fluorescence intensity of K562 cells modified with MAV or PAV. Mean ± SEM, n = 3 independent replicates. d Representative STEM image of polyvalent engineered K562 cells. Bright spots: approximately 10 nm quantum dots. e Schematic illustration showing the rolling and adhesion of engineered K562 cells on C166 cells under flow conditions. f, g Number of engineered K562 cells adhering to C166 cells. K562 cells were modified with MAV or PAV at a density of 1.6 × 107 units/cell and tested under different shear stress conditions (f). K562 cells were modified with different densities of MAV or PAV on the cell surface and tested at the same shear stress condition of 4 dyn/cm2 (g). Mean ± SEM. n = 3 independent replicates. h Representative images showing engineered K562 cells (green) adhering to C166 cells (red). Shear stress: 4 dyn/cm2. Representative images out of 7 images obtained are shown. Source data are provided as a Source Data file.

Back to article page