Fig. 2: Vγ9Vδ2 T cell lytic vesicles are mature and loaded with granzyme B.
From: Modulation of lytic molecules restrain serial killing in γδ T lymphocytes
Anti-granzyme B (a–c) or anti-chondroitin sulfate (d, e) labelling of cell sections from isolated γδD4 or γδD14 T cells or isolated IL2-activated NKD4 cells from the same donors, imaged by transmission electron microscopy. 20 cells from the cell sections were imaged for each donor. a Representative immuno-EM sections labelled with anti-granzyme B followed by protein A gold. Scale bars: 0.5 µm. b Number of gold particles (granzyme B) per granule for the different conditions, the bar plots represent the medians. c Quantification of the area of the dense core granules, the bar plots represent the medians (b, c, γδD4 n = 304 granules, γδD14 n = 264 granules, NKD4 n = 311 granules), the data are pooled from 3 donors. d Representative immuno-EM sections labelled with anti-chondroitin sulfate followed by protein A gold. Scale bars: 0.5 µm. e Number of gold particles (chondroitin sulfate) per granule for the different conditions, the bar plots represent the medians (γδD4 n = 240 granules, γδD14 n = 163 granules, NKD4 n = 50 granules), the data are pooled from 3 donors (γδ4, γδD14) and 1 donor (NKD4). b, c, e The individual dots represent one granule and they are colour-coded according to donor. All analysis by Kruskal–Wallis test.
