Fig. 6: Vγ9Vδ2 T cells segregate granzyme B from perforin in different lytic vesicles.
From: Modulation of lytic molecules restrain serial killing in γδ T lymphocytes
a Representative Airyscan microscopy images of γδD4 or NKD4 cells from the same donors prepared by immunofluorescence labelling against granzyme B, perforin and CD107a. Scale bars: 2 µm. b Proportion of colocalized voxels of granzyme B with perforin (GrzB-Prf) or perforin with granzyme B (Prf-GrzB) in CD107a+ vesicles of γδD4 T cells or NKD4 cells. Square boxes are the means for each donor (n = 5 donors, each ≥15 cells). c Representative Airyscan microscopy images of γδD4 or γδD14 T cells from the same donors prepared by immunofluorescence labelling against granzyme B, perforin and CD107a. Scale bars: 2 µm. d Proportion of colocalized voxels of granzyme B with perforin (GrzB-Prf) or perforin with granzyme B (Prf-GrzB) in CD107a+ vesicles of γδD4 or γδD13/14 T cells. Square boxes are the means for each donor (n = 9 donors, each ≥15 cells). e, f Correlation between the sum intensity of granzyme B (e) or perforin (f) in the CD107a+ vesicles with the on-chip percentage of granzyme B-mediated killing of γδD4 T cells (upon HMBPP or not) from the same donors (n = 7). g Correlation between the proportion of colocalized voxels of granzyme B with perforin (b and d) with the on-chip percentage of granzyme B-mediated killing of γδD4 T cells (upon HMBPP or not) from the same donors (n = 7). h Correlation between the proportion of colocalized voxels of granzyme B with perforin (b and d) with the on-chip granzyme B-mediated kills per γδD4 T killer (upon HMBPP or not) from the same donors (n = 7). The lines in e–h are the linear fittings (with HMBPP treatment or not). All are means with standard deviations. Statistics were calculated using two-tailed paired t-test (b and d) and simple linear regression (e–h). ns: not-significant.
