Fig. 2: CIPK1 physically interacts with PYR1/PYL1-6. | Nature Communications

Fig. 2: CIPK1 physically interacts with PYR1/PYL1-6.

From: The CBL1/9-CIPK1 calcium sensor negatively regulates drought stress by phosphorylating the PYLs ABA receptor

Fig. 2

a CIPK1 interaction with PYR1/PYL1-PYL6 in BiFC assay. YFPc, C-terminal portion of YFP. YFPn, N-terminal portion of YFP. Scale bar, 40 μm. b CBL1 anchors the interaction of CIPK1 and PYL4 to the plasma membrane. Tobacco leaf protoplasts injected with different combinations were observed under confocal microscopy. CBL1n-OFP, in which the first 12 N-terminal amino acids of CBL1 are fused to OFP, was used as the plasma-membrane marker (PM Marker). Scale bar, 20 μm. c CIPK1 interaction with PYR1/PYL1-PYL6 in LUC complementation imaging assays (LCI) assay. GUS-nLUC and GUS-cLUC were used as negative controls. d Pull-down assay showing CIPK1 interaction with PYR1/PYL1-PYL6. Anti-GST and Anti-His were used for protein detection. e Co-IP assay showing the interaction of CIPK1 with PYL1 and PYL4. The experiments were repeated three times (a–e) with similar results.

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