Fig. 1: Design and characterization of CoV-S-2P displayed on I53-dn5. | Nature Communications

Fig. 1: Design and characterization of CoV-S-2P displayed on I53-dn5.

From: Nanoparticle display of prefusion coronavirus spike elicits S1-focused cross-reactive antibody response against diverse coronavirus subgenera

Fig. 1

a Computer-generated models of prefusion-stabilized spike trimers (S-2P) from SARS-1, SARS-2, and MERS, and their homotypic display on the icosahedral I53-dn5 nanoparticle displaying 20 trimers. b Trace profiles of S-2P_dn5B trimer and _dn5 nanocage purification by size exclusion chromatography. c ELISA comparing binding of antibodies specific for SARS-1, SARS-2, or MERS_S-2P to soluble trimer (triangles) or dn5 assembly (circles) respectively. d Representative images of CoV-S-2P_dn5 at 50,000× magnification and 2D class averages. Scale bars correspond to 100 nm (representative images) and 20 nm (2D class averages). All experiments were performed at least twice, each repeat with similar results.

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