Fig. 5: β-CoV mosaic particles protect against lethal MERS-CoV challenge. | Nature Communications

Fig. 5: β-CoV mosaic particles protect against lethal MERS-CoV challenge.

From: Nanoparticle display of prefusion coronavirus spike elicits S1-focused cross-reactive antibody response against diverse coronavirus subgenera

Fig. 5

288/330+/+ mice were immunized twice with 10 µg of the specified nanoparticle or soluble trimer. Control mice were immunized with H1_dn5. 4 weeks post-boost, mice were challenged with a lethal dose, 5 × 105 plaque-forming units (p.f.u.) of maM35c4 MERS-CoV. a Following challenge, mice were monitored for weight loss. b, c 5 days post challenge, b lung discoloration (scored as: 0 = no discoloration, 4 = severe discoloration in all lobes) and c lung viral titers were assessed. All groups were compared with H1_dn5 control mice by Kruskal–Wallis analysis of variance (ANOVA) with Dunn’s multiple comparisons test; in (a), the comparison was made at each day post-challenge. *P < 0.05, **P < 0.01. Data depict mean ± s.d. in (a, b) or GMT ± geometric s.d. in (c). In (c), the dotted line represents the assay limit of detection. Challenge experiments were performed once.

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