Fig. 4: Diffusion kinetics of mitochondrial microdomain-specific ROS.
From: All-optical spatiotemporal mapping of ROS dynamics across mitochondrial microdomains in situ
a HyPer7 normalized ratio responses with KillerRed co-expressed in all mitochondrial microdomains. KillerRed photostimulation ranged from 0, 5, 15, 30, 60 and 180 sec. One-way, repeated measure, mixed-effects ANOVA with Geisser-Greenhouse correction and Dunnett post-hoc correction, N = 21 – 55 cells, mean ± SD. b Time to half maximum (T1/2) for microdomain-targeted HyPer7 and KR experiments. Two-way ANOVA with Tukey post-hoc correction, N = 21 – 55 cells, mean ± SD. c Maximal interpolated steady-state H2O2 concentration of mitochondrial microdomains following microdomain-specific KR photogeneration of H2O2. Two-way ANOVA with Tukey post-hoc correction, N = 21 – 55 cells, mean ± SD. d Interpolated apparent H2O2 concentrations in each microdomain from A. Two-way, repeated measure, mixed-effects ANOVA with Geisser-Greenhouse correction and Tukey post-hoc correction, N = 6 – 46 cells, mean ± SD. e Interpolated apparent H2O2 concentrations as in B but depicted as nonlinear fit curves (all R2 > 0.6) across all photostimulation timepoints. *p < 0.05, **p < 0.01, ***p < 0.001. Exact N values are provided in Source Data file.
