Fig. 5: Effect of sHVF18 on transcriptome profiling in mice stimulated with LPS.

a Linkage plot of genes and enriched GO-terms in LPS-treated animals after 8 h identifies a prototypical LPS response. b Ridgeplot showing expression distributions of core enriched transcription factor targets 8 h after LPS treatment (red) and for LPS + sHVF18 (blue) compared to controls (buffer only). IRF and NF-κB are identified as activated transcription factors (positive enrichment score), whereas CDX2 and LHX3 are down-regulated. This regulation is greatly reduced in the presence of sHVF18. c Heatmap of core enriched genes identified in lungs from mice treated with buffer only (control), LPS, or LPS + 50 μg mL⁻¹, after 8 and 20 h for gene sets identified as differentially regulated. Mice stimulated with LPS showed an upregulation of inflammatory genes, which are abrogated by sHVF18 treatment at 8 h and, to a lesser extent, at 20 h. d Venn diagram of the number of overlapping genes differentially expressed (P value < 0.01 and |logFC | > 1 were adjusted for multiple comparisons using the Benjami-Hochberg method) for LPS, LPS + sHVF18, or sHVF18 alone compared to control at 20 h. P values was calculated using a linear two-sided model. Differentially expressed genes were further analyzed for over-enriched GO terms and presented as linkage plots as in a.