Fig. 6: Intravenous administration of sHVF18 ameliorated symptoms of systemic inflammation and hindered the development of severe states of ARDS in pigs. | Nature Communications

Fig. 6: Intravenous administration of sHVF18 ameliorated symptoms of systemic inflammation and hindered the development of severe states of ARDS in pigs.

From: Targeting Toll-like receptor-driven systemic inflammation by engineering an innate structural fold into drugs

Fig. 6

The figure shows results from pigs with acute lung injury and ARDS treated with and without sHVF18. a Schematic overview of the experiment setup. Pigs were anesthetized in mechanical ventilation and monitored continuously using an arterial line and Swan-Ganz catheter. LPS was given intravenously, and treated animals received sHVF18. Figure created with BioRender.com. b–f Hemodynamics, vitals, and pulmonary gas exchange were followed continuously over the time course of the experiment (n = 5 for each group, except for not-treated at 120 minutes, n = 2). b Pulmonary gas exchange as PaO2 FiO2−1 ratio between treated and non-treated pigs. All non-treated animals developed mild to moderate ARDS. A significant increase in cardiac output (c), a significant decrease in urine output (d), and a significantly increased need for inotropic support (e), and a significant increase in lactate levels (f) were seen in the non-treated animals but not in the treated animals indicating a severe stage of systemic inflammation and organ dysfunction in the non-treated pigs. The box in the figures (b–f) extends from the 25th to 75th percentiles. Whiskers represents minimum to maximum values. The line in the middle of the box is plotted at the median. g Images representative of n = 15 samples of hematoxylin and eosin (H&E) histology of healthy controls (n = 5) (left), non-treated (middle) (n = 5) and treated (right) lungs (n = 5). The scale bar in the larger image represents 0.5 mm. The callout shows a magnified portion of the tissue where the scale bar represents 0.2 mm. h The results of cumulative blinded scoring of the histology (n = 5 in each group). Statistically significant differences between non-treated and treated groups were tested with a two-sided Student’s t-test (d), within groups with two-way ANOVA with Šidak´s multiple comparisons test (b, c, e and f) or with one-way ANOVA with Tukey’s multiple comparisons test (h).

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