Table 1 Implant types and target coordinates in the five experiments
From: The medial septum controls hippocampal supra-theta oscillations
Experiment | CA1 implant | CA1 coordinates | MS implant | MS coordinates | Ground location |
|---|---|---|---|---|---|
Urethane anesthetized rat | linear silicon probe (A1x32-6mm-50-177; NeuroNexus Technologies, Ann Arbor, US) | AP: −4.5 mm ML: 3 mm angle: 0° | Buzsáki-type silicon probe (Buzsaki32; NeuroNexus Technologies, Ann Arbor, US) | AP: 0.4 mm ML: 1.6 mm angle: 15° | nuchal muscles |
Urethane anesthetized mice | linear silicon probe (A1x32-6mm-50-177) | AP: −2.2 mm ML: 1.5 mm angle: 0° | single shank custom silicon probe with 32 × 4 square-shaped recording sites184 | AP: 0.6 mm ML: 0.5 mm angle: 8° | neck muscle |
Freely moving mice | linear silicon probe (A1x32-6mm-50-177) or Buzsáki-type silicon probe (Buzsaki32) | AP: −2.5 mm ML: 2 mm angle: 0° | Buzsáki-type silicon probe (Buzsaki32) | AP: 0.9 mm ML: 0.9 mm angle: 12° | cerebellum |
Awake head restrained mice for optogenetic stimulation | 128 channels UCLA silicon microprobes (128 J or 128 A, Masmanidis lab) in both hippocampi | AP: −2.5 mm ML: 2.5 mm angle: 0° | optic fiber (200 µm core diameter, Thorlabs GmbH) | AP: 0.9 mm ML: 0 mm angle: 0° | cerebellum |
Awake head restrained mice for juxtacellular recording | glass electrode filled with 0–3.0% neurobiotin (wt/vol) in 0.5 M NaCl | AP: −2.5 mm ML: 1.7 angle: 10° | glass electrode filled with 2.5–3.0% neurobiotin (wt/vol) or BDA in 0.5 M NaCl | AP: 0.85 mm ML: 0 mm angle: 0° | cerebellum |
