Fig. 5: Ketamine acutely disrupts cell-pair connectivity.
From: Ketamine evoked disruption of entorhinal and hippocampal spatial maps
a Left: Schematic of a spike raster of a stable cell-pair relationship in the baseline epoch. Right: Spiking correlations between stable cell-pairs are conserved across perturbations and result in a diagonal relationship between spiking correlation coefficients (⍴) across two conditions (top right schematic). If a test perturbation breaks the relationship between two neurons, then that results in a non-diagonal relationship between the spiking correlation coefficients (bottom right schematic). b Two example sessions (each row is a session) of cell-pair correlations across different experimental epochs (baseline: trials 1–50, control: trials 51–100, acute ketamine: trials 101–150, late ketamine: trials 251–290, gain change: trials 291–300). To perturb the relationship between visual cues and locomotion, mice ran 10 trials where the gain was 0.5×. We calculated the correlation coefficient of between the smoothed firing rates of the first 50 baseline trials for all pairs of spatially stable (non-interneurons, spatial score >0.2) cells in a session, then examined cell-pairs with a significant correlation (Pearson correlation coefficient, p < 0.05). Each point is a cell-pair; line is the least squares line of the cell pairs. The Pearson correlation (⍴) value for each condition is at the bottom. c Violin plots of cell-pair correlations across different experimental states. All violins have the same area, but the width represents the kernel probability density of the data at different values. Each point (gray) indicates the Pearson correlation (calculated as shown in (b)) of a session. Sessions with >5 stable cell pairs are plotted (n = 27 sessions). Two-sided Wilcoxon matched pairs signed rank test with Sidak correction for multiple comparisons. Control vs acute ketamine: Z = −4.31, p = 0.0001; control vs late ketamine: Z = −4.72, p = 1.4 × 10−5; control vs gain change: Z = 1.02, p = 0.89; acute vs late ketamine: Z = 1.49, p = 0.58; acute ketamine vs gain change: Z = 4.35, p = 8.2 × 10−5; late ketamine vs gain change: Z = 4.51, p = 3.9 × 10−5). Significant comparisons highlighted ****p < 0.001. Source data are provided as a Source data file.
