Fig. 1: Loss of ENDOG represses lipid accumulation in mouse livers and hepatocytes.

a–c Representative electron microscopic images and quantification of lipid size and number in Endog^+/− or Endog^−/− mouse livers after 24 hours of starvation. N: Nuclear; LD: lipid droplet; n = 8mice. d Measurement of total triglycerides in mouse livers after 24 hours of starvation. n = 8 mice. e, f Representative images of BODIPY staining and the quantitative results of lipid area in liver tissue of mice starved for 24 hours. n = 8 mice. g–i Representative images of Nile red staining, the quantitative results of lipid area, and measurement of triglycerides in WT and ENDOG KO HepG2 cells after treatment of 200 μM oleic acid for 24 h. n = 10 independent samples for Nile red staining and 6 for triglyceride measurement. j–l Representative images of Nile red staining, the quantitative results of lipid area, and measurement of triglycerides in control and ENDOG overexpressing HepG2 cells following the treatment of 200 μM oleic acid for 24 h. pK-Myc and ENDOG overexpressing plasmids were transfected for 48 h. n = 4 independent samples for Nile red staining and 5 for triglyceride measurement. Statistical significance was determined by unpaired Student’s t-test (two-tailed) in (b, c, d, f, h, i, k, l); error bars are mean ± SD. Source data and exact P values are provided in a Source data file. *P < 0.05; **P < 0.01; ***P < 0.001; n.s.: no significance.