Fig. 1: Diurnal multi-omics analysis of the mouse liver under time-restricted feeding.
From: Multi-omics profiling reveals rhythmic liver function shaped by meal timing
a A schematic diagram illustrating the workflow of the multi-omics study on the effects of meal timing in the liver from 9-week-old C57BL/6J female mice. The intervention of meal timing lasts for 1 week. Samples were dissected every 4 h for 2 days (n = 48 mice per group). Proteomics, phosphoproteomics and ubiquityl-proteomics (n = 4 mice per time-point), succinyl-proteomics and N-glycosyl-proteomics (n = 2 mice per time-point) were performed and integrated. Data were matched with diurnal lipidomics (n = 4 mice per time point for 7 time points within one diurnal cycle). b Interaction of diurnal molecules in each omics dataset, and summary of identified and quantified molecules in the multi-omics data. Numbers in blue and red circles of Venn diagrams denote the number of rhythmic molecules, while numbers in the gray area denote arrhythmic molecules. Circadian rhythmicity was determined by the algorithms MetaCycle (adjusted P-value < 0.05), RAIN (adjusted P-value < 0.05), and CircaCompare (P-value < 0.05). DRF, day/sleep time-restricted feeding; NRF, night/wake time-restricted feeding; NAPE, N-acylphosphatidyl ethanolamine; EC, endocannabinoid; acyl-AA, acyl amino acids. Source data are provided as a Source data file.
