Fig. 2: Entrainment of the diurnal phosphoproteome by DRF.
From: Multi-omics profiling reveals rhythmic liver function shaped by meal timing
a Expression profiles of phosphorylated proteins in the mouse liver that are diurnal under both DRF and NRF (referred to as dual-cycling phospho-proteins). Average expression levels were log2-transformed and scaled (n = 48 mice per group). b, c Histogram showing the distribution of phase (b) or phase-shift (c) of dual-cycling phospho-proteins in the mouse liver. Locked, phase shift [0, 2 h]; inverted, phase shift [8, 12 h]; numbers denote phase-locked/inverted rhythmic phosphoproteins over total dual-cycling phosphoproteins. d Histogram showing the phase distribution of enriched GO: Biological Process pathways, as measured by phase set enrichment analysis (PSEA, Kuiper test q < 0.05) of hepatic dual-cycling phospho-proteins. e, f Display of the least (e) or the most (f) phase-shifted pathways. Pathway terms are ranked based on the average phase shift and q-values, and the top 5 are displayed. g, h Diurnal profiles of representative phospho-proteins involved in the receptor-mediated endocytosis (g) and regulation of circadian rhythm (h) in the mouse liver. Data are presented as mean values  ±  standard deviation, n = 4 mice per time point for 12 time points. Source data are provided as a Source data file.
