Fig. 4: Clock regulation of fatty acid metabolism is a key feature of the hepatic proteome under DRF.
From: Multi-omics profiling reveals rhythmic liver function shaped by meal timing
a Expression profiles of dual-cycling ubiquityl-proteins in the mouse liver. Average expression levels were log2-transformed and scaled (n = 48 mice per group). b Histogram showing the phase-shift distribution of dual-cycling hepatic ubiquityl-proteins. Locked, phase shift [0, 2 h]; inverted, phase shift [8, 12 h]; numbers denote phase-locked/inverted rhythmic ubiquityl-proteins over total dual-cycling ubiquityl-proteins. c, d Histogram showing the phase-shift distribution (c) and representative pathways (d) of enriched GO: Biological Process pathways, as measured by phase set enrichment analysis (PSEA, Kuiper test q < 0.05) of hepatic dual-cycling ubiquityl-proteins. e Diurnal expression of ubiquitylated (ub-) SLC27A2 in mouse liver. Data are presented as mean values ± standard deviation, n = 4 mice per time point for 12 time points. f–i Sample plot per Omics (f), loading plot displaying the contribution (loading weight) of each feature selected from the first component per Omics in an increasing order of importance from the bottom up (g), circos plot showing the positive (negative) correlation (r > 0.8) between selected features (rhythmic proteins) as indicated by the red (blue) links (h) and clustered image maps of component 1 features (i) from the N-integrative supervised analysis with multi-omics data sampled in the evening (ZT8 and ZT12, n = 16 per group). Diurnal proteins are included for this analysis. Phos.protein, phosphorylated proteins; Ubiq.protein, ubiquitylated proteins. Source data are provided as a Source data file.
