Fig. 5: Integrated analyses of diurnal transcriptome and multi-level proteomes.
From: Multi-omics profiling reveals rhythmic liver function shaped by meal timing
a Histogram showing the phase distribution of enriched rhythmic pathways under DRF or NRF, as measured by PSEA of cycling proteins in livers from DRF or NRF female mice (Kuiper test, q < 0.05). b, c Representative rhythmic pathways in mouse livers under DRF (b) or NRF (c), as shown by the hour of their estimated peak time. d Diurnal profiles of dual-cycling N-glycosyl proteins in the mouse liver. Average expression levels were log2-transformed and scaled (n = 24 mice per group). e Histogram showing the phase-shift distribution of dual-cycling hepatic N-glycosyl proteins. Locked, phase shift [0, 2 h]; inverted, phase shift [8, 12 h]; numbers denote phase-locked/inverted rhythmic N-glycosyl proteins over total dual-cycling N-glycosyl proteins. f Diurnal profiles of N-glycosylated (ng-) APOB and ANGPTL3 in mouse liver, as measured by affinity purification-mass spectrometry. N = 24 mice per group. g Interaction of diurnal succinyl-sites between DRF and NRF. h Diurnal profiles of dual-cycling succinyl-sites in the mouse liver. Average expression levels were log2-transformed and scaled (n = 24 mice per group). i Diurnal profile of succinylation at K644 of HADHA (HADHA_su-K644). CircaCompare method (n = 24 mice per group, P < 0.05, two-sided test). Source data are provided as a Source data file.
